Friday, May 15, 2009

Under ideal growth conditions, E.coli strain BL21(DE3) can express up to 30% of its total protein content from an inducible T7 expression vector....

Since the E.coli cells can express only a maximum of 30% of its total protein content, we first have to figure out the total protein content in the given concentration of cells.


It is given that 5 x 10^9 E.coli cells have a total protein yield of 45 `mug`. The given concentration of the E.coli culture is 4.2 x 10^9 cells/ml. In other words, 1 ml of culture contains 4.2 x 10^9 cells. 


Using the given data on total protein yield,


5 x 10^9 cells contain 45 `mug` ,


4.2 x 10^9 cells will contain a total protein content of: 


`45/(5 xx 10^9) xx 4.2 xx 10^9` =  37.8 `mug`.


So, the given culture contains 37.8 `mug`/ml of protein. However, only 30% of it can be expressed.


Thus, the theoretical protein yield = 30% of 37.8 `mug`/ml = 11.34 `mug`/ml


=  1.1e1 `mug`/ml  (in scientific notation).


Hope this helps. 

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