Since the E.coli cells can express only a maximum of 30% of its total protein content, we first have to figure out the total protein content in the given concentration of cells.
It is given that 5 x 10^9 E.coli cells have a total protein yield of 45 . The given concentration of the E.coli culture is 4.2 x 10^9 cells/ml. In other words, 1 ml of culture contains 4.2 x 10^9 cells.
Using the given data on total protein yield,
5 x 10^9 cells contain 45 ,
4.2 x 10^9 cells will contain a total protein content of:
= 37.8
.
So, the given culture contains 37.8 /ml of protein. However, only 30% of it can be expressed.
Thus, the theoretical protein yield = 30% of 37.8 /ml = 11.34
/ml
= 1.1e1 /ml (in scientific notation).
Hope this helps.
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